Implication of mouse Vps26b-Vps29-Vps35 retromer complex in sortilin trafficking

► We generated vacuolar protein sorting (Vps) 26b knock mice. ► Vps26b-deficient mice showed a severe reduction of Vps35 protein levels and lacked the Vps26b-Vps29-Vps35 retromer complex. ► The levels of sortilin is increased approximately 20% in the Vps26b-deficient mice. ► More p75NTR was immunoprecipitated with sortilin antibody from Vps26b−/− brains than from wild-type brains. ► Vps26b-Vps29-Vps35 retromer complex is implicated in the transport of sortilin from endosomes to the trans-Golgi network (TGN).