Biological characterization and structure based prediction of insulin-like growth factor binding protein-5

► IGFBP-5 structures containing N, L, and C domains were separately modeled. ► The L domain of IGFBP-5 was expressed in Escherichia coli and purified. ► The L domain structure of IGFBP-5 was similar to that of the corepressor of repressor element-1 silencing transcription factor (CoREST) linker. ► The purified L domain existed as a homogenous dimer in glutaraldehyde crosslinking.