Regulated expression of acyl-CoA thioesterases in the differentiation of cultured rat brown adipocytes

► Cytosolic ACOT1 expression dominates in brown preadipocytes. ► ACOT1 is downregulated as the cells differentiate and lipid droplets accumulate. ► Mitochondrial ACOT2 and β-oxidation enzymes increase during differentiation. ► ACOT isoform switching supports fat storage and combustion in brown adipocytes.