Actin and Arp2/3 localize at the centrosome of interphase cells

► Actin was detected at the centrosome with the anti-actin antibody 1C7 that recognizes antiparallel (“lower dimer”) actin dimers. ► Centrosomal actin was found in interphase but not mitotic MDA-MB-231 cells. ► Neither the anti-actin antibody C4 that binds to globular, monomer actin, nor the anti-actin antibody 2G2 that recognizes the nuclear conformation of actin detect actin at the centrosome. ► The Arp2/3 complex transiently localizes at the pericentriolar matrix but not at the centrioles of interphase HEK 293T cells.