MicroRNA expression profile of colon cancer stem-like cells in HT29 adenocarcinoma cell line

► We employ the human HT29 cell line as a model, and purify a population of colon CSCs expressing a CD133 surface phenotype by FACS. ► The CD133+ cells exhibit stem-like features including expression of “stemness” genes as well as self-renewal and differentiation capacity. ► We reveal the miRNA expression signature characteristic of colon cancer stem cells for the first time.