کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10765825 1050601 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Daxx positively modulates β-catenin/TCF4-mediated transcriptional potential
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Daxx positively modulates β-catenin/TCF4-mediated transcriptional potential
چکیده انگلیسی
Constitutive activation of the transcription factor TCF4 activity by mutated APC or β-catenin contributes to cell neoplastic transformation. While numerous proteins were identified to activate TCF4-dependent activity via β-catenin interaction, little is known about factors directly acting on TCF4. Here we report that Daxx binds to TCF4 and potentiates β-catenin/TCF4-mediated transcriptional activation and target gene expression. Binding studies revealed that Daxx-TCF4 interaction is through the C-terminal domain of Daxx and TCF4 segment containing amino acid residue 269-327. Alteration of Daxx levels in cells by overexpression or RNA interference resulted in an increase or decrease of the β-catenin/TCF4-dependent transactivation activity and target gene expression, respectively. Furthermore, TCF4-(269-327) segment acts as a dominantly negative mutant by blocking Daxx-TCF4 interaction and TCF4-mediated transactivation potential. Together, our results suggest that Daxx functions as a positive coregulator in modulating the β-catenin/TCF4-dependent transcriptional potential via TCF4 interaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 386, Issue 4, 4 September 2009, Pages 762-768
نویسندگان
, ,