کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10765825 | 1050601 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Daxx positively modulates β-catenin/TCF4-mediated transcriptional potential
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Daxx positively modulates β-catenin/TCF4-mediated transcriptional potential Daxx positively modulates β-catenin/TCF4-mediated transcriptional potential](/preview/png/10765825.png)
چکیده انگلیسی
Constitutive activation of the transcription factor TCF4 activity by mutated APC or β-catenin contributes to cell neoplastic transformation. While numerous proteins were identified to activate TCF4-dependent activity via β-catenin interaction, little is known about factors directly acting on TCF4. Here we report that Daxx binds to TCF4 and potentiates β-catenin/TCF4-mediated transcriptional activation and target gene expression. Binding studies revealed that Daxx-TCF4 interaction is through the C-terminal domain of Daxx and TCF4 segment containing amino acid residue 269-327. Alteration of Daxx levels in cells by overexpression or RNA interference resulted in an increase or decrease of the β-catenin/TCF4-dependent transactivation activity and target gene expression, respectively. Furthermore, TCF4-(269-327) segment acts as a dominantly negative mutant by blocking Daxx-TCF4 interaction and TCF4-mediated transactivation potential. Together, our results suggest that Daxx functions as a positive coregulator in modulating the β-catenin/TCF4-dependent transcriptional potential via TCF4 interaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 386, Issue 4, 4 September 2009, Pages 762-768
Journal: Biochemical and Biophysical Research Communications - Volume 386, Issue 4, 4 September 2009, Pages 762-768
نویسندگان
Yen-Sung Huang, Hsiu-Ming Shih,