کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10765843 | 1050607 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Single-strand DNA translation initiation step analyzed by Isothermal Titration Calorimetry
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Is single-strand DNA translatable? Since the 60s, the question still remains whether or not DNA could be directly translated into protein. Some discrepancies in the results were reported about functional translation of single-strand DNA but all results converged on a similar behavior of RNA and ssDNA in the initiation step. Isothermal Titration Calorimetry method was used to determine thermodynamic constants of interaction between single-strand DNA and S30 extract of Escherichia coli. Our results showed that the binding was not affected by the nature of the template tested and the dissociation constants were in the same range when ssDNA (Kd = 3.62 ± 2.1 Ã 10â8 M) or the RNA corresponding sequence (Kd = 2.7 ± 0.82 Ã 10â8 M) bearing SD/ATG sequences were used. The binding specificity was confirmed by antibiotic interferences which block the initiation complex formation. These results suggest that the limiting step in translation of ssDNA is the elongation process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 385, Issue 3, 31 July 2009, Pages 296-301
Journal: Biochemical and Biophysical Research Communications - Volume 385, Issue 3, 31 July 2009, Pages 296-301
نویسندگان
Luminita Damian, Claire Marty-Detraves, Mathias Winterhalter, Didier Fournier, Laurent Paquereau,