کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10766127 | 1050624 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differentiation and characterization of embryonic stem cells into three germ layers
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Differentiation and characterization of embryonic stem cells into three germ layers Differentiation and characterization of embryonic stem cells into three germ layers](/preview/png/10766127.png)
چکیده انگلیسی
Embryonic stem cells differentiated on M15 cells have previously been shown to give rise to cells of the mesendodermal and definitive endodermal lineages. Here we demonstrate that neuroectodermal and mesodermal lineages can be derived from ES cells cultured on M15 cells and subsequently subjected to specific culture conditions, as confirmed by the expression of molecular markers. Prospective isolation and microarray analyses showed that neuroectodermal cells expressed anterior-to-posterior, as well as dorso-ventral regional markers, suggesting that this procedure could be used for the induction of cells belonging to a wide variety of neural lineages. Lateral mesoderm and paraxial mesoderm cells were also produced and their gene expression profiles were confirmed by microarray analyses. These results indicate that the M15 cell system provides a valuable tool for generating ES cell-derived lineage-specific cell types belonging to the three germ layers, namely neuroectoderm, mesoderm, and definitive endoderm.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 381, Issue 4, 17 April 2009, Pages 694-699
Journal: Biochemical and Biophysical Research Communications - Volume 381, Issue 4, 17 April 2009, Pages 694-699
نویسندگان
Nobuaki Shiraki, Yuichiro Higuchi, Seiko Harada, Kahoko Umeda, Takayuki Isagawa, Hiroyuki Aburatani, Kazuhiko Kume, Shoen Kume,