کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10766648 1050667 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The cytoplasmic C2A domain of synaptotagmin shows sequence specific interaction with its own mRNA
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The cytoplasmic C2A domain of synaptotagmin shows sequence specific interaction with its own mRNA
چکیده انگلیسی
Synaptotagmin-1 (Syt1) is essential in Ca2+-dependent neurotransmitter release, but its expression regulation is unknown. Here we report that the cytoplasmic Syt1 fragment forms ribonucleoprotein complex by interacting with the 3′ untranslated region (3′UTR) of its own mRNA. Two protein-binding domains, GU15 repeat and GUCAAUG, within the Syt 3′UTR and the C2 domains in Syt1, especially C2A, are essential in this ribonucleoprotein complex formation. Furthermore, in in vitro assay the translation efficiency of Syt1 mRNA was downregulated in presence of 3′UTR. These results demonstrate for the fist time that the soluble fraction of Syt1 can interact with its own mRNA in a highly sequence specific manner.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 373, Issue 4, 5 September 2008, Pages 509-514
نویسندگان
, , , ,