کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10767008 1050694 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Association of chicken zona pellucida glycoprotein (ZP) B1 with ZPC induces formation of ZPB1-ZPC fibrous aggregates containing disulfide-bridged ZPB1 dimer
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Association of chicken zona pellucida glycoprotein (ZP) B1 with ZPC induces formation of ZPB1-ZPC fibrous aggregates containing disulfide-bridged ZPB1 dimer
چکیده انگلیسی
Egg-envelope, a fibrous extracellular matrix, surrounding an oocyte is constructed from ZPC, ZPX2, both of which are secreted from ovarian granulosa cells, and ZPB1 which is secreted from liver cells and transported into ovary in birds. We report here that in vitro incubation of ZPB1 with ZPC spontaneously produced fibrous aggregates of ZPB1-ZPC hetero-complexes, which were visible under optical microscopy and morphologically resembled the aggregates obtained from mechanically decomposed chicken egg-envelope. Formation of such fibrous aggregates depended on ZPC/ZPB1 ratio, and involved ZPB1 dimerization through disulfide cross-linking, which had been found in authentic egg-envelope developed in hen's ovary. Furthermore, addition of excessive amounts of ZPC to ZPB1 produced soluble but high molecular weight hetero-complexes with increased adherence property against polystyrene ELISA plates. Thus, the specific association between ZPB1 and ZPC could play pivotal roles to initiate complex formation of hetero-polymers of ZP proteins in egg-envelope matrix construction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 364, Issue 3, 21 December 2007, Pages 682-688
نویسندگان
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