کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767129 | 1050698 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Leukotriene C4 synthase promoter driven expression of GFP reveals cell specificity
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کلمات کلیدی
AP-2human promyelocytic leukemia cell lineLTC4SHL-60PBSTHP-112-O-tetradecanoylphorbol-13-acetatetPAeGFPGFPC/EBP - C / EBPDMSO - DMSOSp1 - SP1Sp3 - sp3all-trans-retinoic acid - اسید کلرید رتینوئیکExpression - اصطلاحRetinoic acid - رتینوئیک اسیدLeukotriene C4 synthase - سنتاز C4 سنتز لوکوترینPhosphate buffered saline - فسفات بافر شورLeukotriene - لکوترینSpecificity protein 1 - مشخصات پروتئین 1CCAAT/enhancer binding protein - پروتئین اتصال CCAAT / enhancerenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استPromoter - پروموتر
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Leukotriene C4 synthase is a key enzyme in leukotriene biosynthesis. Its gene has been cloned and mapped to mouse chromosome 11. Expression occurs in cells of myeloid origin and also in the choroid plexus, the hypothalamus and the medial eminence of mouse brain. In this study a vector that expresses enhanced green fluorescent protein (eGFP) under the control of the mouse leukotriene C4 synthase promoter was constructed and used to study promoter activity in different cell lines. Specific eGFP expression was observed in human monocytic leukemia (THP-1) and rat basophilic leukemia (RBL-1) myeloid cells which both express leukotriene C4 synthase, but not in human embryonic kidney (HEK293/T) epithelial cells which do not express this enzyme. In the myeloid cells, but not in the epithelial cells, we observed that the leukotriene C4 synthase promoter activity was stimulated by 12-O-tetradecanoylphorbol-13-acetate and all-trans-retinoic acid. In contrast dimethyl sulfoxide did not affect promoter activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 366, Issue 1, 1 February 2008, Pages 80-85
Journal: Biochemical and Biophysical Research Communications - Volume 366, Issue 1, 1 February 2008, Pages 80-85
نویسندگان
Tobias Strid, Mats Söderström, Sven Hammarström,