کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767335 | 1050723 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl Role of myofibril-inducing RNA in cardiac TnT expression in developing Mexican axolotl](/preview/png/10767335.png)
چکیده انگلیسی
The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene ``c”, for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts. Myofibril-inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac troponin T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 357, Issue 1, 25 May 2007, Pages 32-37
Journal: Biochemical and Biophysical Research Communications - Volume 357, Issue 1, 25 May 2007, Pages 32-37
نویسندگان
Gian Franco Sferrazza, Chi Zhang, Pingping Jia, Sharon L. Lemanski, Gagani Athauda, Alyssa Stassi, Kristine Halager, Jennifer A. Maier, Elena Rueda-de-Leon, Amit Gupta, Syamalima Dube, Xupei Huang, Howard M. Prentice, Dipak K. Dube, Larry F. Lemanski,