کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10767341 1050723 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
LMO4 mRNA stability is regulated by extracellular ATP in F11 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
LMO4 mRNA stability is regulated by extracellular ATP in F11 cells
چکیده انگلیسی
LIM only domain protein 4 (LMO4) interacts with many signaling and transcription factors to regulate cellular proliferation, differentiation and plasticity. In Drosophila, mutations in the 3′ untranslated region (UTR) of the homologue dLMO cause a gain of function by increasing mRNA stability. LMO4 3′UTR contains several AU-rich elements (ARE) and is highly conserved among vertebrates, suggesting that RNA destabilizing mechanisms are evolutionarily conserved. Here, we found that extracellular ATP stabilized LMO4 mRNA in F11 cells. The LMO4 3′UTR added to a luciferase reporter markedly reduced reporter activity under basal conditions, but increased activity with ATP treatment. Two ARE motifs were characterized in the LMO4 3′UTR. ATP increased binding of HuD protein to ARE1. ARE1 conferred ATP and HuD-dependent mRNA stabilization. In contrast, sequences flanking ARE2 bound CUGBP1 and ATP destabilized this complex. Thus, our results suggest that ATP modulates recruitment of RNA-binding proteins to the 3′UTR to stabilize LMO4 mRNA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 357, Issue 1, 25 May 2007, Pages 56-61
نویسندگان
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