کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10767360 1050723 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Go but not Gi2 or Gi3 is required for muscarinic regulation of heart rate and heart rate variability in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Go but not Gi2 or Gi3 is required for muscarinic regulation of heart rate and heart rate variability in mice
چکیده انگلیسی
Muscarinic receptor-mediated cardiac parasympathetic activity is essential for regulating heart rate and heart rate variability (HRV). It has not been clear which Gi/Go protein is responsible for these effects. We addressed this question using knockout mice that lack G protein αi2, αi3, or αo specifically. Unlike previously reported, our αo-null mice had significantly more survivors with normal life span. Isolated hearts from αo-null mice demonstrated much less sensitivity to the negative chronotropic effects of the muscarinic agonist carbachol to lower heart rate at baseline and a more profound effect under the stimulation of the β-adrenergic agonist isoproterenol. In the presence of parasympathetic activation indirectly produced by methoxamine, an α1-adrenergic agonist, αo-null mice showed markedly decreased HRV compared with wild-type control mice. These differences in heart rate and HRV were not observed in αi2-null or αi3-null mice. Our findings establish an essential role for αo G protein in the anti-adrenergic effect of carbachol on heart rate regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 357, Issue 1, 25 May 2007, Pages 139-143
نویسندگان
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