کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767401 | 1050723 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a disease-specific model to evaluate endothelial dysfunction in patients with diabetes mellitus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
IL-6MCP-1IL-8sVCAM-1NIDDMsICAM-1Endothelial dysfunction - اختلال عملکرد اندوتلیالInterleukin-8 - اینترلوکین -8interleukin-6 - اینترلوکین ۶non-insulin-dependent diabetes mellitus - دیابت غیر وابسته به انسولینDiabetes mellitus - دیابت قندیEndothelial cells - سلولهای اندوتلیالIn vitro model - مدل in vitromonocyte chemoattractant protein-1 - پروتئین شیمیایی monocyte chemoattractant-1
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Diabetic patients have an increased cardiovascular risk. We propose to characterize the endothelial dysfunction in a disease-specific in vitro model. Human saphenous vein endothelial cells (HSVEC) were isolated from coronary artery bypass patients without and with non-insulin-dependent diabetes mellitus. Growth kinetics and proinflammatory responses (expression of adhesion molecules, cytokines) were documented under non-stimulating conditions. Diabetic HSVEC showed delayed growth kinetics with reduced cell densities of about 40%. During exponential growth of diabetic EC, the surface expression of adhesion molecules was increased 10-fold (p ⩽ 0.05). However, in a monolayer the expression adapted to low levels of non-diabetic EC. In addition, diabetic EC produced significantly more soluble E-selectin, VCAM-1, IL-6 and MCP-1. Our results suggest a link between the pathologically proinflammatory basic state of diabetic EC and the endothelial dysfunction in diabetic disease. Therefore, this in vitro model could be used for investigating early dysfunction and environmental effects on pathological endothelium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 357, Issue 1, 25 May 2007, Pages 308-313
Journal: Biochemical and Biophysical Research Communications - Volume 357, Issue 1, 25 May 2007, Pages 308-313
نویسندگان
Karla Lehle, Frank Haubner, Daniela Münzel, Dietrich E. Birnbaum, Jürgen G. Preuner,