کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10767598 | 1050775 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Actin cytoskeletons regulate the stretch-induced increase of Ca2+ current in human gastric myocytes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Using the whole-cell and single channel recording techniques, the influence of actin cytoskeletons on L-type Ca2+ current was investigated in human gastric smooth muscle cells. In isotonic condition, an actin depolymerizer cytochalasin D (Cyt-D) markedly decreased the whole-cell current (IBa) without changing steady-state voltage dependency and single channel conductance. Intracellular dialysis of phalloidin, an actin polymerizer, significantly increased the IBa. Hypotonic stretch (222 mOsm/L) of the myocytes increased the IBa, and Cyt-D significantly inhibited the IBa increase by the stretch. Phalloidin was without effect on the IBa increase by the stretch. Phalloidin antagonized the Cyt-D inhibition of the stretch-induced IBa increase. Neither heterotrimeric G protein modifiers (GTPγS and GDPβS) nor rho GTPase inhibitor (C3 exoenzyme) influenced the stretch-induced responses. These results reveal that the integrity of the actin cytoskeleton is an important factor which determines the activity of L-type Ca2+ channels and a response to stretch.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 352, Issue 2, 12 January 2007, Pages 503-508
Journal: Biochemical and Biophysical Research Communications - Volume 352, Issue 2, 12 January 2007, Pages 503-508
نویسندگان
Jun Hee Kim, Poong-Lyul Rhee, Tong Mook Kang,