کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10767647 1050794 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of a branched-chain amino-acid transporter SBAT1 (SLC6A15) that is expressed in human brain
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Characterization of a branched-chain amino-acid transporter SBAT1 (SLC6A15) that is expressed in human brain
چکیده انگلیسی
The SLC6 gene family comprises membrane proteins that transport neurotransmitters, amino acids, or osmolytes. We report the first functional characterization of the human SLC6A15 gene, which codes for a sodium-coupled branched-chain amino-acid transporter 1 (SBAT1). SBAT1 expression is specific to the brain. When expressed in Xenopus oocytes, SBAT1 mediated Na+-coupled transport of hydrophobic, zwitterionic α-amino and imino acids. SBAT1 exhibited a strong preference for branched-chain amino acids (BCAA) and methionine (K0.5 80-160 μM). SBAT1 excluded aromatic or charged amino acids, β-amino acids, glycine, and GABA. SBAT1-mediated transport of amino or imino acids was extremely temperature-dependent (Q10 = 9) and was inhibited at acidic pH. PKC activation reduced the plasma-membrane population of SBAT1 protein. SBAT1-mediated transport of BCAA, particularly leucine, may be an important source of amino nitrogen for neurotransmitter synthesis in glutamatergic and GABAergic neurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 3, 25 November 2005, Pages 892-900
نویسندگان
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