کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10767812 1050799 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A 'Swinging Cradle' model for in vitro classification of different types of response elements of a nuclear receptor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A 'Swinging Cradle' model for in vitro classification of different types of response elements of a nuclear receptor
چکیده انگلیسی
Nuclear receptors are hormone-activated transcription factors that bind to specific target sequences termed hormone-response element (HRE). A HRE usually consists of two half-sites (5′-AGGTCA-3′ consensus sequence) arranged as a direct, everted or inverted repeat with variable spacer region. Assignment of a HRE as a direct, everted or inverted repeat is based on its homology to the consensus half-site, but minor variations can make such an assignment confusing. We hypothesize a 'Swinging Cradle' model for HRE classification, whereby the core HRE functions as the “sitting platform” for the NR, and the extra nucleotides at either end act as the “sling” of the Cradle. We show that in vitro binding of the thyroid hormone receptor and 9-cis retinoic acid receptor heterodimer to an everted repeat TRE follows the 'Swinging Cradle' model, whereas the other TREs do not. We also show that among these TREs, the everted repeat mediates the highest biological activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 2, 18 November 2005, Pages 490-497
نویسندگان
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