کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768005 | 1050802 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Meiosis-specific yeast Hop1 protein promotes pairing of double-stranded DNA helices via G/C isochores
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Meiosis-specific yeast Hop1 protein promotes pairing of double-stranded DNA helices via G/C isochores Meiosis-specific yeast Hop1 protein promotes pairing of double-stranded DNA helices via G/C isochores](/preview/png/10768005.png)
چکیده انگلیسی
In eukaryotes, genetic exchange between homologs is facilitated by a tripartite proteinaceous structure called the synaptonemal complex (SC). Several lines of evidence indicate that the genes that encode components of SC are essential for meiotic chromosome pairing and recombination. However, the molecular mechanism by which SC proteins promote these processes is obscure. Here, we report that Saccharomyces cerevisiae Hop1 protein, a component of SC, promotes pairing between two double-stranded DNA helices containing a centrally located G/C isochore. Significantly, pairing was rapid and robust, and required four contiguous G/C base pairs. Using a series of truncated DNA double helices we show that 20Â bp on either side of 8Â bp target G/C sequence is essential for pairing. To our knowledge, Hop1 is the first protein shown to do so from yeast or any other organism. These results indicate that Hop1 protein is likely to play a direct role in meiotic chromosome pairing and recombination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 336, Issue 3, 28 October 2005, Pages 934-941
Journal: Biochemical and Biophysical Research Communications - Volume 336, Issue 3, 28 October 2005, Pages 934-941
نویسندگان
S. Anuradha, Pankaj Tripathi, Kiran Mahajan, K. Muniyappa,