کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768218 | 1050804 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A kinetic study of the mechanism of conversion of α-hydroxyheme to verdoheme while bound to heme oxygenase
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
O2-dependent reactions of the ferric and ferrous forms of α-hydroxyheme complexed with water-soluble rat heme oxygenase-1 were examined by rapid-scan stopped-flow measurements. Ferric α-hydroxyheme reacted with O2 to form ferric verdoheme with an O2-dependent rate constant of 4 Ã 105 Mâ1 sâ1 at pH 7.4 and 9.0. A decrease of the rate constant to 2.8 Ã 105 Mâ1 sâ1 at pH 6.5 indicates that the reaction proceeds by direct attack of O2 on the Ï-neutral radical form of α-hydroxyheme, which is generated by deprotonation of the α-hydroxy group. The reaction of ferrous α-hydroxyheme with O2 yielded ferrous verdoheme in a biphasic fashion involving a new intermediate having absorption maxima at 415 and 815 nm. The rate constants for this two-step reaction were 68 and 145 sâ1. These results show that conversion of α-hydroxyheme to verdoheme is much faster than the reduction of coordinated iron (<1 sâ1) under physiological conditions [Y. Liu, P.R. Ortiz de Montellano, Reaction intermediates and single turnover rate constants for the oxidation of heme by human heme oxygenase-1, J. Biol. Chem. 275 (2000) 5297-5307], suggesting that, in vivo, the conversion of ferric α-hydroxyheme to ferric verdoheme precedes the reduction of ferric α-hydroxyheme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 338, Issue 1, 9 December 2005, Pages 578-583
Journal: Biochemical and Biophysical Research Communications - Volume 338, Issue 1, 9 December 2005, Pages 578-583
نویسندگان
Hiroshi Sakamoto, Kenichi Takahashi, Yuichiro Higashimoto, Saori Harada, Graham Palmer, Masato Noguchi,