کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768274 | 1050806 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hyperosmotic stress stimulates inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate formation independently of bis-diphosphoinositol tetrakisphosphate modulation
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Hyperosmotic stress induces water diffusion out of the cell, resulting in cell shrinkage, and leading to DNA damage, cell cycle arrest, and cytoskeletal reorganization. A previous report showed that low concentrations of sorbitol (200Â mM) could increase up to 25-fold the concentration of InsP8 in animal cells. Here, we investigate the effect of sorbitol (200Â mM) on the inositol 1,4,5-trisphosphate (InsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4) pathway. A 3- to 4-fold increase in InsP3 and InsP4 levels after sorbitol challenge was observed. It was prevented by the phospholipase C inhibitor U-73122 but was insensitive to the MAP kinase inhibitor U0126. We also observed an increase in the free intracellular [Ca2+] and the occurrence of Ca2+ oscillations in response to sorbitol. A hyperosmotic stress could therefore affect the levels of both hyperphosphorylated inositol phosphates and InsP3/InsP4-signalling molecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 336, Issue 1, 14 October 2005, Pages 157-162
Journal: Biochemical and Biophysical Research Communications - Volume 336, Issue 1, 14 October 2005, Pages 157-162
نویسندگان
Xavier Pesesse, Alexandre Leyman, Tomas Luyten, Ludwig Missiaen, Christophe Erneux,