کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768581 | 1050812 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Upregulation of MUC6 mucin gene expression by NFκB and Sp factors
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
To clarify the mechanism underlying regulation of MUC6 expression, we isolated the 5â² flanking region of the MUC6 gene (5â²-MUC6). We determined the transcription start site of the MUC6 gene, and found a TATA box at â35 to â29 bp, a putative NFκB consensus sequence at â173 to â164 bp, and putative Sp family binding sites at â530 to â521 and â847 to â838 bp. The luciferase activity of 5â²-MUC6 gradually decreased with deletion of these sites. NFκB inhibitory factor IκB decreased the luciferase activity, and forced expression of NFκB induced MUC6 transcription. An inhibitor of Sp family binding, mithramycin A, suppressed MUC6 transcripts, and Sp1 and Sp3 overexpression up-regulated them. Binding of Sp family members to their putative sites was confirmed by electrophoretic mobility shift assays. Our results suggest that MUC6 transcription is regulated by NFκB and Sp family members.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 4, 12 August 2005, Pages 1254-1260
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 4, 12 August 2005, Pages 1254-1260
نویسندگان
Hidekazu Sakai, Artit Jinawath, Shoji Yamaoka, Yasuhito Yuasa,