کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10768669 1050813 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of tick periviscerokinin, the first neurohormone of Ixodidae: Single cell analysis by means of MALDI-TOF/TOF mass spectrometry
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Identification of tick periviscerokinin, the first neurohormone of Ixodidae: Single cell analysis by means of MALDI-TOF/TOF mass spectrometry
چکیده انگلیسی
The first peptidergic neurohormone from the ticks Ixodes ricinus and Boophilus microplus has been identified by using a combination of immunocytochemistry and mass spectrometric analysis of single cells. The novel peptide (Ixori-PVK, PALIPFPRV-NH2) shows a high sequence homology with members of the insect periviscerokinin/CAP2b peptides that in insects are involved in the regulation of water balance. The function of this peptide in ticks is still unknown, but these pests consume large amounts of blood in a single blood meal which is a challenge for the regulation of diuretic processes. Thus, the novel peptide may be involved in one of the key physiological processes in ticks. High energy collision-induced dissociation was successfully used to distinguish between Leu/Ile ambiguities in single cell preparations. This is the first successful de novo sequencing of a peptide from single cell preparations of arthropods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 338, Issue 4, 30 December 2005, Pages 1860-1864
نویسندگان
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