کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10768868 1050816 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polyamine sensing during antizyme mRNA programmed frameshifting
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Polyamine sensing during antizyme mRNA programmed frameshifting
چکیده انگلیسی
A key regulator of cellular polyamine levels from yeasts to mammals is the protein antizyme. The antizyme gene consists of two overlapping reading frames with ORF2 in the +1 frame relative to ORF1. A programmed +1 ribosomal frameshift occurs at the last codon of ORF1 and results in the production of full-length antizyme protein. The efficiency of frameshifting is proportional to the concentration of polyamines, thus creating an autoregulatory circuit for controlling polyamine levels. The mRNA recoding signals for frameshifting include an element 5′ and a pseudoknot 3′ of the shift site. The present work illustrates that the ORF1 stop codon and the 5′ element are critical for polyamine sensing, whereas the 3′ pseudoknot acts to stimulate frameshifting in a polyamine independent manner. We also demonstrate that polyamines are required to stimulate stop codon readthrough at the MuLV redefinition site required for normal expression of the GagPol precursor protein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 338, Issue 3, 23 December 2005, Pages 1478-1489
نویسندگان
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