کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10769090 | 1050819 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Plasminogen/plasmin regulates α-enolase expression through the MEK/ERK pathway
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Previously we have demonstrated that both plasminogen (Plg) and plasmin (Pla) regulate the expression of the transcription factors c-FOS and EGR-1 [L.P. De Sousa, B.S. Brasil, B.M. Silva, M.H. Freitas, S.V. Nogueira, P.C. Ferreira, E.G. Kroon, C.A. Bonjardim, Plasminogen/plasmin regulates c-fos and egr-1 expression via the MEK/ERK pathway, Biochem. Biophys. Res. Commun. 329 (2005) 237-245]. Here we show that Plg activates the mitogen-activated protein kinases MEK and ERK which leads to α-enolase (α-ENO) gene expression not only in fibroblasts, but also in peripheral blood mononuclear cells. The α-ENO mRNA accumulation was apparent three hours post-Plg treatment and remained elevated out to 28 h, a process that seems to require both de novo protein synthesis and active gene transcription. Pla mimics Plg-stimulated α-ENO expression through its serine protease activity, suggesting that conversion of Plg to active Pla is required. Pharmacological and genetic blockade of MEK caused inhibition of α-ENO mRNA accumulation, implicating MEK/ERK as the transduction pathway that leads to α-ENO expression upon Plg stimulation. Furthermore, Plg stimulated DNA binding activity of the transcription factors activator-protein 1 and early growth response gene-1 to their cognate regulatory sequences at α-ENO promoter. Altogether, our data show that Plg/Pla regulates α-ENO expression through the MEK/ERK pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 4, 2 December 2005, Pages 1065-1071
Journal: Biochemical and Biophysical Research Communications - Volume 337, Issue 4, 2 December 2005, Pages 1065-1071
نویسندگان
Lirlândia P. Sousa, Breno M. Silva, Bruno S.A.F. Brasil, Sarah V. Nogueira, Paulo C.P. Ferreira, Erna G. Kroon, Kanefusa Kato, Cláudio A. Bonjardim,