کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10769254 1050820 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glucocorticoids increase C/EBPβ activity in the lung epithelium via phosphorylation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Glucocorticoids increase C/EBPβ activity in the lung epithelium via phosphorylation
چکیده انگلیسی
Glucocorticoids are widely prescribed anti-inflammatory drugs used for the treatment of many inflammatory lung disorders. However, much still remains unknown about their molecular mechanisms of action. We have previously shown that glucocorticoid-induced transcription in the lung epithelial cell line NCI-H441 is mediated via C/EBP sites in the promoters of target genes, and is likely to involve the transcription factors C/EBPβ and C/EBPδ. Here, we report that C/EBPβ is the most active C/EBP-factor in both human and mouse lung epithelium and that glucocorticoids induce DNA binding of C/EBPβ in cultured primary mouse lung epithelial cells. Mechanistic studies in H441 cells revealed that glucocorticoids, acting via the glucocorticoid receptor, increase C/EBPβ binding starting 10 min after stimulation. The mechanism is independent of de novo protein synthesis and involves phosphorylation of C/EBPβ at Thr235. Together this shows that glucocorticoids increase DNA-binding activity of C/EBPβ via post-translational mechanism(s) involving phosphorylation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 334, Issue 2, 26 August 2005, Pages 638-645
نویسندگان
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