کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10769757 1050825 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway
چکیده انگلیسی
Several protein kinases have been shown to be involved in spermatogenesis. Recently, a novel subfamily of serine/threonine kinases has been isolated whose expression is limited to testis. Here, we report the fifth family member, named TSSK5, which encodes a 328 amino acid protein. RT-PCR analysis showed that TSSK5 is exclusively expressed in human testis. We isolated cAMP responsive element binding protein (CREB), a TSSK5 interacting protein via yeast two-hybrid system. The in vitro kinase assay showed that TSSK5 phosphorylated CREB at Ser-133. Using a CRE reporter system, we found that TSSK5 could stimulate the CREB/CRE responsive pathway in Hek293 cells. These results suggest that this kinase may be involved in spermatogenesis through phosphorylating CREB and then stimulating the CREB/CRE responsive pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 3, 5 August 2005, Pages 742-749
نویسندگان
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