کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10769767 | 1050825 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The non-selective cation-permeable channel TRPC3 is a tetrahedron with a cap on the large cytoplasmic end
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The non-selective cation-permeable channel TRPC3 is a tetrahedron with a cap on the large cytoplasmic end The non-selective cation-permeable channel TRPC3 is a tetrahedron with a cap on the large cytoplasmic end](/preview/png/10769767.png)
چکیده انگلیسی
TRPC3 plays important roles in neuronal differentiation and immune cell maturation by mediating the cationic current in response to phospholipase C activation, Ca2+ depletion, and diacylglycerol stimulation. Here, we purified the TRPC3 channel using a glycosylated tetramer and observed the structure using electron microscopy. Negatively stained specimens demonstrate homogeneous protein particles containing an internal cavity-like structure. These particle images were picked up by automated pick-up programs, aligned, and classified by the growing neural gas network method. Similarly oriented projections were averaged to decrease the signal-to-noise ratio. The averaged images progress from the top view to the side views, which are representative of their raw images. The top view confirmed the hypothesis of a four-domain structure, and the side view demonstrates a large cytoplasmic domain with a capped structure at the bottom, which is near a predicted locus of ion release. The total image of the protein is a blunt-edged trapezoid of 200Â ÃÂ 200Â ÃÂ 235Â Ã
. This large dimension of TRPC3 is also supported by the Stokes radius (92Â Ã
) obtained from gel filtration chromatography.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 3, 5 August 2005, Pages 768-777
Journal: Biochemical and Biophysical Research Communications - Volume 333, Issue 3, 5 August 2005, Pages 768-777
نویسندگان
Kazuhiro Mio, Toshihiko Ogura, Yuji Hara, Yasuo Mori, Chikara Sato,