کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10769885 1050826 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The CXCR1 tail mediates β1 integrin-dependent cell migration via MAP kinase signaling
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The CXCR1 tail mediates β1 integrin-dependent cell migration via MAP kinase signaling
چکیده انگلیسی
In this study, we examined how IL-8 induces leukocyte migration on major β1 integrin ligands derived from the extracellular matrix protein fibronectin. We assessed individual contributions of signaling by IL-8 receptors by transfection of CXCR1 and CXCR2 into rat basophilic leukemia (RBL) cells and human monocytic THP-1 cells. CXCR1 expressing cells migrated on the fibronectin ligands for α4β1 and α5β1 integrins in response to IL-8, whereas CXCR2 expressing cells did not. RBL cells expressing the chimeric CXCR1 receptor containing the cytoplasmic tail of CXCR2 had greatly blunted migration, while cells expressing the CXCR2 chimera with the tail of CXCR1 had augmented migration. Last, inhibitors of p38 and JNK MAP kinases blocked IL-8-induced migration in CXCR1(+) cells. We conclude that IL-8 stimulated β1 integrin-mediated leukocyte migration on fibronectin through CXCR1 is dependent on the C-terminal cytoplasmic domain of CXCR1 and subsequent p38 and JNK MAPK signaling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 332, Issue 1, 24 June 2005, Pages 117-125
نویسندگان
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