کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10770462 | 1050833 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of single amino acid substitutions at the predicted coiled-coil or hydrophobic region on the self-assembly of Ï29 replication protein, gp1
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of single amino acid substitutions at the predicted coiled-coil or hydrophobic region on the self-assembly of Ï29 replication protein, gp1 Effects of single amino acid substitutions at the predicted coiled-coil or hydrophobic region on the self-assembly of Ï29 replication protein, gp1](/preview/png/10770462.png)
چکیده انگلیسی
Gp1, the product of one of the essential genes of Ï29 replication, is an RNA binding protein and self-associates to form large complexes. Furthermore, gp1 suppresses the synthesis of Ï29 DNA polymerase and primer protein in the post-transcriptional process. In this report, we have employed seven variants with single amino acid substitutions to analyze the self-assembly of gp1. Using chemical cross-linking and sedimentation assays, amino acid substitutions within the predicted coiled-coil or hydrophobic region were shown to strongly affect the formation of large complexes, suggesting that these two regions were required for the self-assembly of gp1. The self-association of gp1 was suggested to be necessary for the efficient binding to RNA and the translational repression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 331, Issue 4, 17 June 2005, Pages 1310-1316
Journal: Biochemical and Biophysical Research Communications - Volume 331, Issue 4, 17 June 2005, Pages 1310-1316
نویسندگان
Kazuya Hashiyama, Ari Takeuchi, Osamu Makino,