کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10770568 | 1050834 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hypoxic stress enhances osteoclast differentiation via increasing IGF2 production by non-osteoclastic cells
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کلمات کلیدی
Osteoclast - استخوانکاه، استئوکلاستDifferentiation - تفکیکDNA microarray - ریزآرایه ای DNA stromal cell-derived factor 1 - عامل مشتق از استروما 1insulin-like growth factor 2 - فاکتور رشد مانند انسولین 2Hypoxia - هیپوکسیreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسreceptor activator of nuclear factor-κB ligand - گیرنده گیرنده لیگاند فاکتور هسته ای κB
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Development of bone depends on a continuous supply of bone-degrading osteoclasts. Although several factors such as cytokines and integrins have been shown to be important for osteoclast recruitment, their mechanism of action is poorly understood. In this study, we demonstrated the enhancement of osteoclast formation by hypoxia and investigated the molecular mechanisms involved. Primary mouse bone marrow cells were cultured in normoxic and hypoxic conditions, and RNA was prepared from each group of cells. Total RNAs were applied to a DNA microarray analysis and then RT-PCR was performed to confirm the microarray data. The most interesting finding of our microarray analysis was upregulation of insulin-like growth factor 2 (IGF2) and stromal cell-derived factor 1 (SDF1) under hypoxic conditions. RT-PCR analysis revealed that IGF2 expression was markedly upregulated in the non-osteoclastic cells. The addition of exogenous IGF2 increased the number of osteoclastic TRAP-positive multinuclear cells formed under normoxic conditions, whereas the addition of exogenous SDF1 did not change osteoclast formation. These results suggest that the upregulation of IGF2 derived from non-osteoclastic cells might be a crucial factor for osteoclast differentiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 328, Issue 4, 25 March 2005, Pages 885-894
Journal: Biochemical and Biophysical Research Communications - Volume 328, Issue 4, 25 March 2005, Pages 885-894
نویسندگان
Hayato Fukuoka, Mineyoshi Aoyama, Ken Miyazawa, Kiyofumi Asai, Shigemi Goto,