کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10770968 | 1050837 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The role of glycosylation in the function of a 48-kDa glycoprotein from carrot
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Carrot extracellular dermal glycoprotein (EDGP) may play an important role in plant defense systems and in signal transduction. Our experiments show that differences in pI values of EDGP isoforms are caused by differences in amino acid sequence and not by heterogeneity in phosphorylation. The binding affinity of native EDGP for a 4-kDa hormone-like peptide from soybean was approximately one-third that of deglycosylated EDGP, and deglycosylation of EDGP caused complete loss of its ability to inhibit xyloglucan-specific endo-β-1,4-glucanase. Experiments using tunicamycin-treated carrot cell cultures showed that glycosylation is essential for correct EDGP folding and secretion, and that tunicamycin does not affect EDGP gene transcription.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 328, Issue 1, 4 March 2005, Pages 144-149
Journal: Biochemical and Biophysical Research Communications - Volume 328, Issue 1, 4 March 2005, Pages 144-149
نویسندگان
Chengwei Shang, Hidenori Sassa, Hisashi Hirano,