کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10771023 | 1050837 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reversal of experimental diabetes by multiple bone marrow transplantation
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Reversal of experimental diabetes by multiple bone marrow transplantation Reversal of experimental diabetes by multiple bone marrow transplantation](/preview/png/10771023.png)
چکیده انگلیسی
Therapeutic utility of bone marrow transplantation in diabetic patients to overcome deficient β-cell population is an attractive proposal. However, the status of bone marrow stem cells (BMSCs) under hyperglycemia is not known. In the present study, we investigated the status of BMSCs in experimental-diabetic mice and demonstrated the rescue of experimental diabetes by multiple diabetic bone marrow transplantation. Our flow-cytometry analysis for CD34+, CD45+, flk1+, c-kit+, and CD34+CD45+ revealed that BMSC reserve remains unaffected under sustained hyperglycemia. We found that single injection of diabetic bone marrow cells (â¼106) resulted in reduction and stabilization of moderate hyperglycemia. However, multiple injections at regular intervals led to restoration of stabilized normoglycemia during a 30 day follow-up. Reversal of diabetes was evidenced by disappearance of hyperglycemia, normal intra-peritoneal glucose tolerance test, and histology and morphometry of pancreas. The present study thus demonstrates that diabetic bone marrow retains its stemness and potential to induce pancreatic regeneration on transplantation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 328, Issue 1, 4 March 2005, Pages 318-325
Journal: Biochemical and Biophysical Research Communications - Volume 328, Issue 1, 4 March 2005, Pages 318-325
نویسندگان
Meenal Banerjee, Anil Kumar, Ramesh R. Bhonde,