کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10771173 | 1050839 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Agonist-induced tyrosine phosphorylation of Gq/G11α requires the intact structure of membrane domains
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Agonist-induced tyrosine phosphorylation of Gq/G11α requires the intact structure of membrane domains Agonist-induced tyrosine phosphorylation of Gq/G11α requires the intact structure of membrane domains](/preview/png/10771173.png)
چکیده انگلیسی
Stimulation of receptors coupled to Gq/G11 protein may induce phosphorylation on a tyrosine residue of the α subunit of this G protein, which is an essential event for Gq/G11 activation. Here we observed that in HEK293 cells stably expressing high levels of thyrotropin-releasing hormone (TRH) receptors and G11α protein the maximal tyrosine phosphorylation of Gq/G11α was reached within 10 min of TRH stimulation and then it faded away at longer time periods of agonist exposure. The Gq/G11α protein levels did not change during this treatment. Incubation of intact cells with β-cyclodextrin (βCD) for 40 min prior to hormone exposure significantly decreased the rapid transient tyrosine phosphorylation. Subsequent replenishment of cholesterol levels reversed the former negative effect of βCD. Isolation of caveolin-enriched, detergent-resistant membrane domains indicated destruction of these structures in βCD-treated cells. These data indicate that the preserved integrity of plasma membrane domains/caveolae is required for complete agonist-induced phosphorylation of Gq/G11α.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 328, Issue 2, 11 March 2005, Pages 526-532
Journal: Biochemical and Biophysical Research Communications - Volume 328, Issue 2, 11 March 2005, Pages 526-532
نویسندگان
Petr MatouÅ¡ek, Dana Durchánková, Ivana Å vandová, Jiri Novotny, Petr Svoboda,