کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10771364 1050841 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intracellular cAMP controls a physical association of V-1 with CapZ in cultured mammalian endocrine cells
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Intracellular cAMP controls a physical association of V-1 with CapZ in cultured mammalian endocrine cells
چکیده انگلیسی
V-1, an ankyrin repeat protein with the activity to control tyrosine hydroxylase (TH) gene expression and transmitter release in PC12D cells, associates with CapZ, an actin capping protein, and thereby regulates actin polymerization in vitro. In this study, immunoprecipitation and Western blot analysis showed that V-1 was physically associated with CapZ-β in PC12D transfectants overexpressing V-1. These proteins were co-localized in the soma of Purkinje cells of rat cerebellum as assayed by immunohistochemistry. Furthermore, in the V-1 transfectants, the amount of CapZ which physically associated with V-1 was steeply reduced at 2 h after treatment with forskolin, but was thereafter increased to reach its initial level at 12 h after forskolin-treatment. These results suggest that the association of V-1 with CapZ is controlled by a cAMP-dependent signalling pathway probably to play a functional role in the regulatory mechanism of actin dynamics in the endocrine system and the central nervous system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 331, Issue 1, 27 May 2005, Pages 181-186
نویسندگان
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