کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10771502 | 1050842 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
First report of a novel plant lysozyme with both antifungal and antibacterial activities
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: First report of a novel plant lysozyme with both antifungal and antibacterial activities First report of a novel plant lysozyme with both antifungal and antibacterial activities](/preview/png/10771502.png)
چکیده انگلیسی
A novel lysozyme exhibiting antifungal activity and with a molecular mass of 14.4 kDa in SDS-polyacrylamide gel electrophoresis was isolated from mung bean (Phaseolus mungo) seeds using a procedure that involved aqueous extraction, ammonium sulfate precipitation, ion exchange chromatography on CM-Sephadex, and high-performance liquid chromatography on POROS HS-20. Its N-terminal sequence was very different from that of hen egg white lysozyme. Its pI was estimated to be above 9.7. The specific activity of the lysozyme was 355 U/mg at pH 5.5 and 30 °C. The lysozyme exhibited a pH optimum at pH 5.5 and a temperature optimum at 55 °C. It is reported herein, for the first time, that a novel plant lysozyme exerted an antifungal action toward Fusarium oxysporum, Fusarium solani, Pythium aphanidermatum, Sclerotium rolfsii, and Botrytis cinerea, in addition to an antibacterial action against Staphylococcus aureus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 3, 18 February 2005, Pages 820-827
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 3, 18 February 2005, Pages 820-827
نویسندگان
Shaoyun Wang, Tzi Bun Ng, Tianbao Chen, Dongyun Lin, Jinhong Wu, Pingfan Rao, Xiuyun Ye,