کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10771679 | 1050843 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro evidence for the recognition of 8-oxoGTP by Ras, a small GTP-binding protein
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Oxygen radicals attack guanine bases in DNA but they also attack cytoplasmic GTP forming 8-oxoGTP. The presence of 8-oxoGTP in cytoplasm is evidenced by the fact that cells contain MutT/MTH1 which hydrolyze 8-oxoGTP into 8-oxoGMP. In this study, the interaction between 8-oxoGTP and Ras, a small GTP-binding protein, was tested in vitro, and the action of 8-oxoGTP was compared to that of GTP. When purified Ras was treated with 8-oxoGTPγS, Ras was activated, as indicated by the enhanced binding of Ras with Raf-1. GTPγS also activated Ras but 8-oxoGTPγS had a much more potent effect. In lysates of human embryo kidney 293 cells, 8-oxoGTPγS activated not only Ras but also the downstream effectors of the Ras-ERK pathway, i.e., Raf-1 and ERK1/2. In contrast to Ras, other small GTP-binding proteins, Rac1 and Cdc42, were inactivated by 8-oxoGTPγS, whereas both of these proteins were activated by GTPγS, indicating that the biological natures of 8-oxoGTP and GTP differ. These results suggest the possibility that 8-oxoGTP is not a simple by-product but a functional molecule transmitting an oxidative signal to small GTP-binding proteins like Ras.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 1, 4 February 2005, Pages 342-348
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 1, 4 February 2005, Pages 342-348
نویسندگان
Sun-Hee Yoon, Jin-Won Hyun, Jinhee Choi, Eun-Young Choi, Hee-Joon Kim, Su-Jae Lee, Myung-Hee Chung,