کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10771902 | 1050845 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ca2+ signaling down-regulates TGF-β1 gene expression in CD4+ T cells
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
In the immune system, TGF-β1 exerts two major functions, anti-inflammatory and immuno-suppressive effects. This work aims to investigate the molecular mechanisms involved in the regulation of the TGF-β1 gene expression in CD4+ T cells. The TGF-β1 gene expresses three transcripts of 2.5, 1.9, and 1.4 kb. The 1.9 kb mRNA which has the highest translation activity was the major transcript. The relationship between T cell receptor (TCR) stimulation and the expression of the gene was investigated. TCR stimulation with a low dose of antigen peptide enhanced the gene expression, whereas a higher dose suppressed the expression. TCR stimulation activates PKC/MAPK and Ca2+ signaling pathways. PMA increased the gene expression, whereas ionomycin decreased the gene expression, markedly. The results indicate that Ca2+ signaling down-regulates TGF-β1 gene expression. The molecular regulation of TGF-β1 gene expression is unique when comparing to other cytokine genes which are generally activated by Ca2+ signaling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 2, 11 February 2005, Pages 494-499
Journal: Biochemical and Biophysical Research Communications - Volume 327, Issue 2, 11 February 2005, Pages 494-499
نویسندگان
Masako Kohyama, Yasuhito Yasogi, Naoko Nakano, Wataru Ise, Shuichi Kaminogawa, Nobumichi Hozumi,