کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10795334 | 1052572 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pigment organisation in the membrane-intrinsic major light-harvesting complex of Amphidinium carterae: Structural characterisation of the peridinins and chlorophylls a and c2 by resonance Raman spectroscopy and from sequence analysis
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کلمات کلیدی
DBSFCPDDXLN2PESCHLsPCPFWHMIntramolecular charge-transfer - انتقال اتمی بین مولکولیEnergy transfer - انتقال انرژیSequence analysis - تجزیه و تحلیل توالیTransient absorption - جذب گذراDouble bonds - دو اوراق قرضهDiadinoxanthin - دیادینوکسانتینResonance Raman - رامان رزونانسStructure - ساختار یا سازهPotential energy surface - سطح انرژی بالقوهFull-width Half Maximum - عرض کامل نصف حداکثرICT - فناوری اطلاعات و ارتباطات CARS - ماشین هاLight-harvesting complex - مجتمع جمع آوری نورLUTS - می آیدLiquid nitrogen - نیتروژن مایعHoop - هوپCarotenoids - کاروتنوئیدهاChlorophylls - کلروفیل
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The structures and environments of the protein-bound peridinins (Pers) and chlorophylls (Chls) a/c2 in the membrane-intrinsic major light-harvesting complex of the dinoflagellate Amphidinium carterae (LHCAmph) are characterised using resonance Raman (RR) spectroscopy with 11 excitation wavelengths, at 77 K. The excitation-dependent variation in the CC stretching mode (ν1) suggests the presence of three Pers with conjugation lengths over 8 double bonds (dBs), and one diadinoxanthin, between 413.7 and 528.7 nm. Two Perred species are revealed on excitation at 550 and 560 nm. These Perred species exhibit anomalously low ν1 values, together with notable resonant enhancement of lactone ring-breathing and -deformation modes. To discern protein-specific effects, the RR spectra are compared to that of Per in polar (acetonitrile), polarisable (toluene) and polar-protic (ethanol) solvents. Resonantly enhanced lactone, ring-breathing (942 cmâ 1) and ring-deformation (~ 650 cmâ 1), modes are identified both in solution, and in the protein, and discussed in the context of the mixing of the S1 and S2 states, and formation of the intramolecular charge-transfer (ICT) state. In the Chl-absorbing region, two sets of Chl c2's, and (at least) six Chl a's can be differentiated. With a pigment ratio of 5-6 (Chl a):2 (Chl c2):5-6 (Per):1 Ddx determined from the fit to the RT absorption and 77 K RR spectra, sequence comparison of LHCAmp to LHCII, and the diatom LHC, fucoxanthin-chlorophyll-a/c-protein (FCP), a template for the conserved pigment binding sites is proposed, to fill the paucity of structural information in the absence of a crystal structure for LHCAmph.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1847, Issue 10, October 2015, Pages 1187-1199
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1847, Issue 10, October 2015, Pages 1187-1199
نویسندگان
Lavanya Premvardhan, Bruno Robert, Roger G. Hiller,