Generation, characterization and crystallization of a cytochrome c1-subunit IV fused cytochrome bc1 complex from Rhodobacter sphaeroides

► We have generated R. sphaeroides mutant bc1 complex c1-14Gly-IV-6His. ► The purified mutant complex shows more structural stability. ► Crystals (5.5 Å) containing four subunits were obtained.