کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10796040 1052655 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetic activation of yeast mitochondrial d-lactate dehydrogenase by carboxylic acids
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Kinetic activation of yeast mitochondrial d-lactate dehydrogenase by carboxylic acids
چکیده انگلیسی
Aerobically grown yeast cells express mitochondrial lactate dehydrogenases that localize to the mitochondrial inner membrane. The d-lactate dehydrogenase is a zinc-flavoprotein with high acceptor specificity for cytochrome c, that catalyzes the oxidation of d-lactate into pyruvate. In this paper, we show that mitochondrial respiratory rate in phosphorylating or non-phosphorylating conditions with d-lactate as substrate is stimulated by carboxylic acids. This stimulation does not affect the yield of oxidative phosphorylation. Furthermore, this stimulation lies at the level of the d-lactate dehydrogenase. It is non-competitive, hyperbolic and its dimension is directly related to the number of carboxylic groups on the activator. The physiological meaning of such a regulation is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1777, Issue 10, October 2008, Pages 1283-1288
نویسندگان
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