Interactions of Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 with membranes at cold and ambient temperatures-Surface morphology and single-molecule force measurements show phase separation, and reveal tertiary and quaternary associations

► Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 are expressed during cold stress. ► Both dehydrins associate with lipid monolayers and bilayers, and cause domain formation. ► Single molecule force microscopy shows both proteins to undergo tertiary compaction on membranes. ► TsDHN-2 also undergoes potential dimerization. ► Results support that these dehydrins stabilize plant membranes during cold stress.