Binding of moesin and ezrin to membranes containing phosphatidylinositol (4,5) bisphosphate: A comparative study of the affinity constants and conformational changes

► We studied the affinity of moesin with PIP2-containing LUV and GUV membranes. ► We investigated the secondary structure of moesin and ezrin in solution by FTIR spectroscopy. ► We proved that ezrin and moesin interaction with PIP2-LUVs increased sensitivity to chymotrypsin. ► We proved that interaction with PIP2-LUVs leads a change in the secondary structure of the proteins.