Model membrane studies for characterization of different antibiotic activities of lipopeptides from Pseudomonas

► Underinvestigated Pseudomonas-lipopeptides were screened for antibiotic activity. ► Lipodipeptide SB-253514 possesses significant antibacterial activity. ► All CLPs showed high membrane binding affinity and insertion tendency. ► Membrane disintegration is not key for the antibiotic mode of action. ► The data imply a targeted mechanism for the antimicrobial activity of SB-253514.