Transmembrane and extramembrane contributions to membrane protein thermal stability: Studies with the NaChBac sodium channel

► The NaChBac channel is very resistant to thermal denaturation. ► Differential thermal stability profiles of extramembrane and transmembrane residues. ► Membrane protein constructs with sequentially removed extramembranous residues. ► Synchrotron Radiation Circular Dichroism spectroscopy detection of subtle effects.