Structural characterization of the interaction of the polyene antibiotic Amphotericin B with DODAB bicelles and vesicles

► A new optical parameter for the relative quantitation of amphiphilic-bound monomeric AmB. ► Calculation of maximum monomeric AmB bound to DODAB bicelles and vesicles. ► Optical absorption, DSC and ESR show AmB aggregates are in water in DODAB dispersions. ► ESR spectra analysis clearly shows that AmB binds at DODAB bicelle edges. ► ESR analysis discussed here could be used with other drugs or other AmB carriers.