The interaction of cannabinoid receptor agonists, CP55940 and WIN55212-2 with membranes using solid state 2H NMR

► Lipid membrane effects of two cannabinoid agonists, WIN55212-2 and CP55940, are investigated. ► DPPC lipid membrane with specific deuterium labels provides extra details for solid state 2H NMR studies. ► The cannabinoid agonists induce lipid lateral phase separation and promote microdomain formation. ► The cooperative process of the lipid acyl chain C-2H segment may be modulated by the cannabinoids. ► The agonists lower the lipid phase transition temperature and modulate the chain order distinctively.