Multivalent protein binding in carbohydrate-functionalized monolayers through protein-directed rearrangement and reorientation of glycolipids at the air-water interface

► Con A binding to glycolipid-containing binary monolayers at the air-water interface. ► Protein-directed spatial rearrangement and reorientation of glycolipids. ► Formation of multivalent protein binding and enhancement of binding affinity. ► Immobilization of rearranged binary monolayers for subsequent biosensing.