Influence of detergents on the activity of the ABC transporter LmrA

► We investigated the influence of 40 detergents on the ABC transporter LmrA. ► Solubilization efficiency, oligomeric state and functionality were analyzed. ► LmrA displayed a preference for zwitterionic detergents. ► In detergent solution FC-16 was superior, while DDM displayed modest activity. ► In proteoliposomes, DDM-reconstituted LmrA was superior to FC-16.