Structural and functional analyses of PpENA1 provide insights into cation binding by type IID P-type ATPases in lower plants and fungi

►Amino acids on MHs 4, 6 and 8 of PpENA1 contribute to cation binding. ►Cation binding sites of PpENA1 differ from those of fungal homologs. ►Comparative modeling enables the engineering of cation binding residues.